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KD-Validated Anti-EOGT Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-EOGT Rabbit Monoclonal Antibody AGI1202
    Western blotting analysis using anti-EOGT antibody (Cat#61408). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EOGT antibody (Cat#61408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). EOGT, EGF domain specific O-linked N-acetylglucosamine transferase.
    detail
  • WB - KD-Validated Anti-EOGT Rabbit Monoclonal Antibody AGI1202
    Western blotting analysis using anti-EOGT antibody (Cat#61408). EOGT expression in wild type (WT) and EOGT shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EOGT antibody (Cat#61408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-EOGT Rabbit Monoclonal Antibody AGI1202
    Flow cytometric analysis of EOGT expression in HT-1080 cells using EOGT antibody (Cat#61408, 1:2,000). Green, isotype control; red, EOGT.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q5NDL2
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3585
Calculated MW Predicted, 62 kDa , observed, 55 kDa
Gene Name EOGT
Aliases EOGT; EGF Domain Specific O-Linked N-Acetylglucosamine Transferase; AER61; EGF Domain-Specific O-Linked N-Acetylglucosamine (GlcNAc) Transferase; EGF Domain-Specific O-Linked N-Acetylglucosamine Transferase; Extracellular O-Linked N-Acetylglucosamine Transferase; AER61 Glycosyltransferase; FLJ33770; C3orf64; EOGT1; Chromosome 3 Open Reading Frame 64; EGF-O-GlcNAc Transferase; EC 2.4.1.255; C3ORF64; AOS4
Immunogen A synthesized peptide derived from human AER61
Additional Information
Gene ID 285203
Other Names EGF domain-specific O-linked N-acetylglucosamine transferase, 2.4.1.255, Extracellular O-linked N-acetylglucosamine transferase, EOGT, AER61, C3orf64, EOGT1
Protein Information
Name EOGT
Synonyms AER61, C3orf64, EOGT1
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.
Cellular Location Endoplasmic reticulum lumen {ECO:0000255|PROSITE- ProRule:PRU10138}
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1202
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