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KD-Validated Anti-LMAN1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-LMAN1 Rabbit Monoclonal Antibody AGI1247
    Western blotting analysis using anti-LMAN1 antibody (Cat#AGI1247). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LMAN1 antibody (Cat#AGI1247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-LMAN1 Rabbit Monoclonal Antibody AGI1247
    Western blotting analysis using anti-LMAN1 antibody (Cat#AGI1247). LMAN1 expression in wild type (WT) and LMAN1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LMAN1 antibody (Cat#AGI1247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-LMAN1 Rabbit Monoclonal Antibody AGI1247
    Flow cytometric analysis of LMAN1 expression in HepG2 cells using LMAN1 antibody (Cat#AGI1247, 1:2,000). Green, isotype control; red, LMAN1.
    detail
  • ICC - KD-Validated Anti-LMAN1 Rabbit Monoclonal Antibody AGI1247
    Immunocytochemical staining of HepG2 cells with LMAN1 antibody (Cat#AGI1247, 1:1,000). Nuclei were stained blue with DAPI; LMAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P49257
Clonality Monoclonal
Isotype Rabbit IgG
Calculated MW 57549 Da
Gene Name LMAN1
Aliases LMAN1; Lectin, Mannose Binding 1; ERGIC53; ERGIC-53; MCFD1; FMFD1; F5F8D; MR60; Gp58; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53; ER-Golgi Intermediate Compartment 53 KDa Protein; Intracellular Mannose-Specific Lectin MR60; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Lectin, Mannose-Binding, 1; Lectin Mannose-Binding 1; GP58
Immunogen A synthesized peptide derived from human LMAN1
Additional Information
Gene ID 3998
Other Names Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D
Protein Information
Name LMAN1
Synonyms ERGIC53, F5F8D
Function Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Cellular Location Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Ubiquitous..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1247
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