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KD-Validated Anti-MCM3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-MCM3 Rabbit Monoclonal Antibody AGI1256
    Western blotting analysis using anti-MCM3 antibody (Cat#61488). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM3 antibody (Cat#61488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-MCM3 Rabbit Monoclonal Antibody AGI1256
    Western blotting analysis using anti-MCM3 antibody (Cat#61488). MCM3 expression in wild type (WT) and MCM3 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MCM3 antibody (Cat#61488, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-MCM3 Rabbit Monoclonal Antibody AGI1256
    Immunocytochemical staining of HepG2 cells with MCM3 antibody (Cat#61488, 1:1,000). Nuclei were stained blue with DAPI; MCM3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P25205
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2490
Calculated MW Predicted, 91 kDa , observed, 100 kDa
Gene Name MCM3
Aliases MCM3; Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; DNA Replication Licensing Factor MCM3; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3; Cervical Cancer Proto-Oncogene 5; DNA Replication Factor MCM3; HRlf Beta Subunit; EC 3.6.4.12; HCC5; P1.H; RLFB
Immunogen A synthesized peptide derived from human MCM3
Additional Information
Gene ID 4172
Other Names DNA replication licensing factor MCM3, 3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3 (HGNC:6945)
Protein Information
Name MCM3 (HGNC:6945)
Function Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (Probable).
Cellular Location Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1256
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