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KD-Validated Anti-PPM1G Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PPM1G Rabbit Monoclonal Antibody AGI1269
    Western blotting analysis using anti-PPM1G antibody (Cat#AGI1269). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1G antibody (Cat#AGI1269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PPM1G Rabbit Monoclonal Antibody AGI1269
    Western blotting analysis using anti-PPM1G antibody (Cat#AGI1269). PPM1G expression in wild type (WT) and PPM1G shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PPM1G antibody (Cat#AGI1269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PPM1G Rabbit Monoclonal Antibody AGI1269
    Flow cytometric analysis of PPM1G expression in HepG2 cells using PPM1G antibody (Cat#AGI1269, 1:2,000). Green, isotype control; red, PPM1G.
    detail
  • ICC - KD-Validated Anti-PPM1G Rabbit Monoclonal Antibody AGI1269
    Immunocytochemical staining of HepG2 cells with PPM1G antibody (Cat#AGI1269, 1:1,000). Nuclei were stained blue with DAPI; PPM1G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O15355
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2585
Calculated MW Predicted, 59 kDa , observed, 72 kDa
Gene Name PPM1G
Aliases PPM1G; Protein Phosphatase, Mg2+/Mn2+ Dependent 1G; PP2CG; Protein Phosphatase Magnesium-Dependent 1 Gamma; Protein Phosphatase 1G ; Protein Phosphatase 1C; EC 3.1.3.16; PP2C-Gamma; PP2CGAMMA; Protein Phosphatase 1G (Formerly 2C), Magnesium-Dependent, Gamma Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G; Protein Phosphatase 2C, Gamma Isoform; Protein Phosphatase 2C Isoform Gamma; PP2C, Gamma; PP2Cgamma; PPP2CG; PPM1C
Immunogen A synthesized peptide derived from human PPM1G
Additional Information
Gene ID 5496
Other Names Protein phosphatase 1G, 3.1.3.16, Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, PPM1G, PPM1C
Protein Information
Name PPM1G
Synonyms PPM1C
Cellular Location Cytoplasm. Membrane; Lipid-anchor
Tissue Location Widely expressed. Most abundant in testis, skeletal muscle, and heart
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1269
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