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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody   

KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody AGI1297
    Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody AGI1297
    Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297). Cysteinyl-tRNA synthetase 1 expression in wild-type (WT) and cysteinyl-tRNA synthetase 1 (CARS1) knockdown (KD) HSHC cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody AGI1297
    Flow cytometric analysis of Cysteinyl-tRNA synthetase 1 expression in HT-1080 cells using anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297, 1:2,000). Green, isotype control; red, Cysteinyl-tRNA synthetase 1.
    detail
  • ICC - KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody AGI1297
    Immunocytochemical staining of HT-1080 cells with anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#AGI1297, 1:1,000). Nuclei were stained blue with DAPI; Cysteinyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P49589
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2790
Calculated MW Predicted, 85 kDa; observed, 75 kDa
Gene Name CARS1
Aliases CARS1; Cysteinyl-TRNA Synthetase 1; CARS; Cysteine TRNA Ligase1,Cytoplasmic; Cysteine--TRNA Ligase, Cytoplasmic; Cysteinyl-TRNA Synthetase; EC 6.1.1.16; Cysteine Translase; MGC:11246; EC 6.1.1; MCDDBH; CYSRS; CysRS; MDBH
Immunogen A synthesized peptide derived from human CARS
Additional Information
Gene ID 833
Other Names Cysteine--tRNA ligase, cytoplasmic, 6.1.1.16, Cysteinyl-tRNA synthetase, CysRS, CARS1 (HGNC:1493), CARS
Protein Information
Name CARS1 (HGNC:1493)
Synonyms CARS
Function Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Cellular Location Cytoplasm.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1297
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