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KD-Validated Anti-Adiponectin receptor 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Adiponectin receptor 1 Rabbit Monoclonal Antibody AGI1312
    Western blotting analysis using anti-Adiponectin receptor 1 antibody (Cat#61573). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adiponectin receptor 1 antibody (Cat#61573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Adiponectin receptor 1 Rabbit Monoclonal Antibody AGI1312
    Western blotting analysis using anti-Adiponectin receptor 1 antibody (Cat#61573). Adiponectin receptor 1 expression in wild type (WT) and Adiponectin receptor 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Adiponectin receptor 1 antibody (Cat#61573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Adiponectin receptor 1 Rabbit Monoclonal Antibody AGI1312
    Flow cytometric analysis of Adiponectin receptor 1 expression in C2C12 cells using Adiponectin receptor 1 antibody (Cat#61573, 1:2000). Green, isotype control; red, Adiponectin receptor 1.
    detail
  • ICC - KD-Validated Anti-Adiponectin receptor 1 Rabbit Monoclonal Antibody AGI1312
    Immunocytochemical staining of C2C12 cells with Adiponectin receptor 1 antibody (Cat#61573, 1:1,000). Nuclei were stained blue with DAPI; Adiponectin receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q96A54
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3150
Calculated MW Predicted, 43 kDa, observed, 52 kDa
Gene Name ADIPOR1
Aliases ADIPOR1; Adiponectin Receptor 1; PAQR1; ACDCR1; Progestin And AdipoQ Receptor Family Member 1; Progestin And AdipoQ Receptor Family Member I; Adiponectin Receptor Protein 1; TESBP1A; CGI-45; CGI45
Immunogen A synthesized peptide derived from human ADIPOR1
Additional Information
Gene ID 51094
Other Names Adiponectin receptor protein 1, Progestin and adipoQ receptor family member 1, Progestin and adipoQ receptor family member I, ADIPOR1 (HGNC:24040)
Protein Information
Name ADIPOR1 (HGNC:24040)
Function Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295). Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein Note=Localized to the cell membrane and intracellular organelles
Tissue Location Widely expressed (PubMed:16044242). Highly expressed in heart and skeletal muscle (PubMed:12802337). Expressed at intermediate level in brain, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes (PubMed:12802337). Weakly expressed in colon, thymus and small intestine (PubMed:12802337)
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1312
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