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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody   

KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody AGI1313
    Western blotting analysis using anti-AKR1C1/AKR1C2 antibody (Cat#AGI1313). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1C1/AKR1C2 antibody (Cat#AGI1313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody AGI1313
    Western blotting analysis using anti-AKR1C1 antibody (Cat#AGI1313). AKR1C1 expression in wild type (WT) and AKR1C1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-AKR1C1 antibody (Cat#AGI1313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody AGI1313
    Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using AKR1C1/AKR1C2 antibody (Cat#AGI1313, 1:2,000). Green, isotype control; red, AKR1C1/AKR1C2.
    detail
  • ICC - KD-Validated Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody AGI1313
    Immunocytochemical staining of Hela cells with AKR1C1/AKR1C2 antibody (Cat#AGI1313, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q04828/ P52895
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3175
Calculated MW Predicted, 37 kDa; Observed, 35 kDa
Gene Name AKR1C1/AKR1C2
Aliases AKR1C1/2; Aldo-Keto Reductase Family 1 Member C1/2; DDH; DD1/2; HAKRC; MBAB; DDH1/2; Dihydrodiol Dehydrogenase 1/2; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase; High-Affinity Hepatic Bile Acid-Binding Protein; Chlordecone Reductase Homolog HAKRC; Dihydrodiol Dehydrogenase 1/2; HBAB; Aldo-Keto Reductase Family 1, Member C1/2 (Dihydrodiol Dehydrogenase 1; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase); Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; Type II 3-Alpha-Hydroxysteroid Dehydrogenase; 20 Alpha-Hydroxysteroid Dehydrogenase; 20-Alpha-Hydroxysteroid Dehydrogenase; Hepatic Dihydrodiol Dehydrogenase; Dihydrodiol Dehydrogenase 1/2; Aldo-Keto Reductase C; Indanol Dehydrogenase; 20-ALPHA-HSD; 20-Alpha-HSD; EC 1.1.1.112; EC 1.1.1.209; EC 1.1.1.210; EC 1.1.1.357; EC 1.1.1.149; 2-ALPHA-HSD; EC 1.1.1.51; EC 1.1.1.53; EC 1.1.1.62; EC 1.3.1.20; EC 1.1.1.; EC 1.1.1; DD1/DD2; H-37; C9
Immunogen A synthetic peptide of human AKR1C1 / AKR1C2
Additional Information
Protein Information
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$ 149.00
$ 499.00
Cat# AGI1313
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