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KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody AGI1328
    Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#61591). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody AGI1328
    Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#61591). DNA damage inducible transcript 3 expression in wild-type (WT) and DNA damage inducible transcript 3 (DDIT3) knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody AGI1328
    Flow cytometric analysis of DNA damage inducible transcript 3 expression in HepG2 cells using anti-DNA damage inducible transcript 3c antibody (Cat#61591, 1:2,000). Green, isotype control; red, DNA damage inducible transcript 3.
    detail
  • ICC - KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody AGI1328
    Immunocytochemical staining of HepG2 cells with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:1,000). Nuclei were stained blue with DAPI; DNA damage inducible transcript 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P35638
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2955
Calculated MW Predicted, 19 kDa; observed, 27 kDa
Gene Name DDIT3
Aliases DDIT3; DNA Damage Inducible Transcript 3; GADD153; CHOP10; CHOP; C/EBP Zeta; Growth Arrest And DNA Damage-Inducible Protein GADD153; CCAAT/Enhancer-Binding Protein Homologous Protein; DNA Damage-Inducible Transcript 3 Protein; C/EBP-Homologous Protein 10; Alternative DDIT3 Protein; AltDDIT3; CHOP-10; Growth Arrest And DNA-Damage-Inducible Gene; DDIT3 Upstream Open Reading Frame Protein; DNA-Damage-Inducible Transcript 3; C/EBP Homologous Protein; C/EBP-Homologous Protein; C/EBPzeta; DDIT-3; CEBPZ
Immunogen A synthesized peptide derived from human DDIT3
Additional Information
Gene ID 1649
Other Names DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, DDIT3, CHOP, CHOP10, GADD153
Protein Information
Name DDIT3
Synonyms CHOP, CHOP10, GADD153
Function Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed:15322075, PubMed:15775988, PubMed:19672300). Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed:15322075, PubMed:15775988). Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (By similarity). Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (By similarity). Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L (PubMed:15775988, PubMed:17709599, PubMed:20876114, PubMed:22761832). Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) (PubMed:18940792, PubMed:19672300, PubMed:20829347). Together with ATF4, mediates ER- mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress (By similarity). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity (PubMed:16434966). Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response (By similarity). Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling (By similarity).
Cellular Location Cytoplasm. Nucleus Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1328
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