Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody   

KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody AGI1329
    Western blotting analysis using anti-fibroblast growth factor receptor 3 antibody (Cat#61593). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fibroblast growth factor receptor 3 antibody (Cat#61593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody AGI1329
    Western blotting analysis using anti-fibroblast growth factor receptor 3 antibody (Cat#61593). Fibroblast growth factor receptor 3 expression in wild-type (WT) and fibroblast growth factor receptor 3 (FGFR3) knockdown (KD) 293T cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-fibroblast growth factor receptor 3 antibody (Cat#61593, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody AGI1329
    Flow cytometric analysis of Fibroblast growth factor receptor 3 expression in C2C12 cells using anti-Fibroblast growth factor receptor 3 antibody (Cat#61593, 1:2,000). Green, isotype control; red, Fibroblast growth factor receptor 3.
    detail
  • ICC - KD-Validated Anti-Fibroblast Growth Factor Receptor 3 Rabbit Monoclonal Antibody AGI1329
    Immunocytochemical staining of C2C12 cells with anti-Fibroblast growth factor receptor 3 antibody (Cat#61593, 1:1,000). Nuclei were stained blue with DAPI; Fibroblast growth factor receptor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P22607
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2965
Calculated MW Predicted, 88 kDa; observed, 98 kDa
Gene Name FGFR3
Aliases FGFR3; Fibroblast Growth Factor Receptor 3; JTK4; CD333; CEK2; EC 2.7.10.1; FGFR-3; ACH; Achondroplasia, Thanatophoric Dwarfism; Fibroblast Growth Factor Receptor 3-S; Hydroxyaryl-Protein Kinase; Tyrosine Kinase JTK4; CD333 Antigen; HSFGFR3EX; EC 2.7.10
Immunogen A synthesized peptide derived from human FGFR3
Additional Information
Gene ID 2261
Other Names Fibroblast growth factor receptor 3, FGFR-3, 2.7.10.1, CD333, FGFR3, JTK4
Protein Information
Name FGFR3
Synonyms JTK4
Function Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Endoplasmic reticulum. Note=The activated receptor is rapidly internalized and degraded. Detected in intracellular vesicles after internalization of the autophosphorylated receptor [Isoform 3]: Secreted.
Tissue Location Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells.
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 149.00
$ 499.00
Cat# AGI1329
Size:
Quantity:
Availability:
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaMexicoNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"