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KD-Validated Anti-AP1G1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-AP1G1 Rabbit Monoclonal Antibody AGI1342
    Western blotting analysis using anti-AP1G1 antibody (Cat#AGI1342). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP1G1 antibody (Cat#AGI1342, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-AP1G1 Rabbit Monoclonal Antibody AGI1342
    Western blotting analysis using anti-AP1G1 antibody (Cat#AGI1342). AP1G1 expression in wild type (WT) and AP1G1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-AP1G1 antibody (Cat#AGI1342, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-AP1G1 Rabbit Monoclonal Antibody AGI1342
    Flow cytometric analysis of AP1G1 expression in HepG2 cells using AP1G1 antibody (Cat#AGI1342, 1:2,000). Green, isotype control; red, AP1G1.
    detail
  • ICC - KD-Validated Anti-AP1G1 Rabbit Monoclonal Antibody AGI1342
    Immunocytochemical staining of HepG2 cells with AP1G1 antibody (Cat#AGI1342, 1:1,000). Nuclei were stained blue with DAPI; AP1G1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O43747
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3655
Calculated MW Predicted, 91 kDa, observed, 98 kDa
Gene Name AP1G1
Aliases AP1G1; Adaptor Related Protein Complex 1 Subunit Gamma 1; Gamma1-Adaptin; CLAPG1; ADTG; Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain; Adaptor Related Protein Complex 1 Gamma 1 Subunit; Golgi Adaptor HA1/AP1 Adaptin Subunit Gamma-1; Adaptor Protein Complex AP-1 Subunit Gamma-1; AP-1 Complex Subunit Gamma-1; Clathrin-Associated/Assembly/Adaptor Protein, Large, Gamma 1; Clathrin Assembly Protein Complex 1 Gamma Large Chain; Adaptor-Related Protein Complex 1, Gamma 1 Subunit; Adapter-Related Protein Complex 1 Subunit Gamma-1; Adaptor-Related Protein Complex 1 Subunit Gamma-1; Golgi Adaptor HA1/AP1 Adaptin Gamma Subunit; Testicular Tissue Protein Li 21; Gamma Adaptin; USRISD
Immunogen A synthesized peptide derived from human AP1G1
Additional Information
Gene ID 164
Other Names AP-1 complex subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, AP1G1, ADTG, CLAPG1
Protein Information
Name AP1G1
Synonyms ADTG, CLAPG1
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed:15758025).
Cellular Location Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin-coated pit. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex (PubMed:12773381). Co-localizes with AFTPH/aftiphilin in the cytoplasm (PubMed:15758025).
Tissue Location Widely expressed.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1342
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