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KD-Validated Anti-Exocyst Complex Component 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Exocyst Complex Component 2 Rabbit Monoclonal Antibody AGI1348
    Western blotting analysis using anti-exocyst complex component 2 antibody (Cat#AGI1348). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-exocyst complex component 2 antibody (Cat#AGI1348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Exocyst Complex Component 2 Rabbit Monoclonal Antibody AGI1348
    Western blotting analysis using anti-exocyst complex component 2 antibody (Cat#AGI1348). Exocyst complex component 2 expression in wild type (WT) and exocyst complex component 2 (EXOC2) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-exocyst complex component 2 antibody (Cat#AGI1348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Exocyst Complex Component 2 Rabbit Monoclonal Antibody AGI1348
    Flow cytometric analysis of Exocyst complex component 2 expression in HepG2 cells using anti-Exocyst complex component 2 antibody (Cat#AGI1348, 1:2,000). Green, isotype control; red, Exocyst complex component 2.
    detail
  • ICC - KD-Validated Anti-Exocyst Complex Component 2 Rabbit Monoclonal Antibody AGI1348
    Immunocytochemical staining of HepG2 cells with anti-Exocyst complex component 2 antibody (Cat#AGI1348, 1:1,000). Nuclei were stained blue with DAPI; Exocyst complex component 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q96KP1
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3030
Calculated MW Predicted, 104 kDa; observed, 98 kDa
Gene Name EXOC2
Aliases EXOC2; Exocyst Complex Component 2; SEC5L1; Sec5; Exocyst Complex Component Sec5; FLJ11026; SEC5-Like 1 (S. Cerevisiae); SEC5-Like 1; NEDFACH; Sec5p; SEC5
Immunogen A synthesized peptide derived from human EXOC2
Additional Information
Gene ID 55770
Other Names Exocyst complex component 2, Exocyst complex component Sec5, EXOC2, SEC5, SEC5L1
Protein Information
Name EXOC2
Synonyms SEC5, SEC5L1
Function Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Cellular Location Midbody, Midbody ring Note=Recruitment to the midbody does not require RALA, nor RALB (PubMed:18756269). Colocalizes with CNTRL/centriolin at the midbody ring (PubMed:16213214).
Tissue Location Widely expressed with highest levels in brain and placenta.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1348
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