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KD-Validated Anti-CAPG Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CAPG Rabbit Monoclonal Antibody AGI1351
    Western blotting analysis using anti-CAPG antibody (Cat#61619). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-CAPG Rabbit Monoclonal Antibody AGI1351
    Western blotting analysis using anti-CAPG antibody (Cat#61619). CAPG expression in wild-type (WT) and CAPG knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CAPG antibody (Cat#61619, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-CAPG Rabbit Monoclonal Antibody AGI1351
    Flow cytometric analysis of CAPG expression in C2C12 cells using anti-CAPG antibody (Cat#61619, 1:2,000). Green, isotype control; red, CAPG.
    detail
  • ICC - KD-Validated Anti-CAPG Rabbit Monoclonal Antibody AGI1351
    Immunocytochemical staining of C2C12 cells with anti-CAPG antibody (Cat#61619, 1:1,000). Nuclei were stained blue with DAPI; CAPG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P40121
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3045
Calculated MW Predicted, 38 kDa; observed, 38 kDa
Gene Name CAPG
Aliases CAPG; Capping Actin Protein, Gelsolin Like; MCP; AFCP; Capping Protein (Actin Filament), Gelsolin-Like; Macrophage-Capping Protein; Epididymis Secretory Protein Li 66; Actin-Regulatory Protein CAP-G; Actin Regulatory Protein CAP-G; Gelsolin-Like Capping Protein; Macrophage Capping Protein; HEL-S-66
Immunogen A synthesized peptide derived from human CAPG
Additional Information
Gene ID 822
Other Names Macrophage-capping protein, Actin regulatory protein CAP-G, CAPG, AFCP, MCP
Protein Information
Name CAPG
Synonyms AFCP, MCP
Function Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Cellular Location Nucleus. Cytoplasm Melanosome. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P24452}. Cell projection, ruffle {ECO:0000250|UniProtKB:P24452}. Note=In macrophages, may be predominantly cytoplasmic. Nuclear localization was observed in fibroblasts. In macrophages, present at the membrane-cytoplasm interface. In activated macrophages, concentrated in the ruffles of the leading lamellipodia. {ECO:0000250|UniProtKB:P24452}
Tissue Location Macrophages and macrophage-like cells.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1351
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