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KD-Validated Anti-Atg16L1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Atg16L1 Rabbit Monoclonal Antibody AGI1364
    Western blotting analysis using anti-Atg16L1 antibody (Cat#61641). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Atg16L1 antibody (Cat#61641, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Atg16L1 Rabbit Monoclonal Antibody AGI1364
    Western blotting analysis using anti-Atg16L1 antibody (Cat#61641). Atg16L1 expression in wild type (WT) and Atg16L1 shRNA knockdown (KD) 293T cells with 30 µg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Atg16L1 antibody (Cat#61641,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Atg16L1 Rabbit Monoclonal Antibody AGI1364
    Flow cytometric analysis of Atg16L1 expression in HT-1080 cells using Atg16L1 antibody (Cat#61641, 1:2,000). Green, isotype control; red, Atg16L1.
    detail
  • ICC - KD-Validated Anti-Atg16L1 Rabbit Monoclonal Antibody AGI1364
    Immunocytochemical staining of HT-1080 cells with Atg16L1 antibody (Cat#61641, 1:1,000). Nuclei were stained blue with DAPI; Atg16L1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q676U5
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB665 Species Reactivity
Calculated MW Predicted, 68 kDa , observed, 68 kDa
Gene Name ATG16L1
Aliases ATG16L1; Autophagy Related 16 Like 1; ATG16A; APG16L; WDR30; Autophagy-Related Protein 16-1; FLJ10035; ATG16L; ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae); ATG16 Autophagy Related 16-Like (S. Cerevisiae); APG16 Autophagy 16-Like (S. Cerevisiae); ATG16 Autophagy Related 16-Like 1; WD Repeat Domain 30; APG16-Like 1; APG16L Beta; IBD104C, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog C; AUT-Like 1, Cysteine; Endopeptidase; APG4 Autophagy 4 Homolog C; EC 3.4.22.-; EC 3.4.22; APG4-C
Immunogen A synthesized peptide derived from human ATG16L1
Additional Information
Gene ID 55054
Other Names Autophagy-related protein 16-1, APG16-like 1, ATG16L1 {ECO:0000303|PubMed:17200669, ECO:0000312|HGNC:HGNC:21498}
Protein Information
Name ATG16L1 {ECO:0000303|PubMed:17200669, ECO:0000312|HGNC:HGNC:21498}
Function Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP) (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576, PubMed:29317426, PubMed:30778222, PubMed:33909989). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non- canonical signaling (PubMed:29317426, PubMed:30778222). During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8 (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8 (PubMed:33909989). Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus (By similarity). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:22749352, PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2 (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Note=Recruited to omegasomes membranes by WIPI2 (By similarity). Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures (By similarity). Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity). Also localizes to discrete punctae along the ciliary axoneme (By similarity). Upon activation of non-canonical autophagy, recruited to single-membrane endolysosomal compartments (PubMed:29317426). Under starved conditions, the ATG12-ATG5-ATG16L1 complex is translocated to phagophores driven by RAB33B (PubMed:32960676). {ECO:0000250|UniProtKB:Q8C0J2, ECO:0000269|PubMed:29317426, ECO:0000269|PubMed:32960676}
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$ 149.00
$ 499.00
Cat# AGI1364
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