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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody   

KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody AGI1386
    Western blotting analysis using anti-ubiquitin specific peptidase 11 antibody (Cat#AGI1386). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitin specific peptidase 11 antibody (Cat#AGI1386, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody AGI1386
    Western blotting analysis using anti-ubiquitin specific peptidase 11 antibody (Cat#AGI1386). Ubiquitin specific peptidase 11 expression in wild type (WT) and ubiquitin specific peptidase 11 (USP11) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ubiquitin specific peptidase 11 antibody (Cat#AGI1386, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody AGI1386
    Flow cytometric analysis of Ubiquitin specific peptidase 11 expression in HeLa cells using anti-Ubiquitin specific peptidase 11 antibody (Cat#AGI1386, 1:2,000). Green, isotype control; red, Ubiquitin specific peptidase 11.
    detail
  • ICC - KD-Validated Anti-Ubiquitin Specific Peptidase 11 Rabbit Monoclonal Antibody AGI1386
    Immunocytochemical staining of HeLa cells with anti-Ubiquitin specific peptidase 11 antibody (Cat#AGI1386, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P51784
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3310
Calculated MW Predicted, 110 kDa; observed, 110 kDa
Gene Name USP11
Aliases USP11; Ubiquitin Specific Peptidase 11; UHX1; Ubiquitin Carboxyl-Terminal Hydrolase 11; Deubiquitinating Enzyme 11 3 4 Ubiquitin Thioesterase 11; Ubiquitin Carboxyl-Terminal Hydrolase, X-Linked; Ubiquitin-Specific Processing Protease 11; Ubiquitin-Specific-Processing Protease 11; Ubiquitin Specific Protease 11; Ubiquitin Thiolesterase 11; EC 3.4.19.12; EC 3.1.2.15
Immunogen A synthesized peptide derived from human USP11
Additional Information
Other Names Ubiquitin carboxyl-terminal hydrolase 11, 3.4.19.12, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11, USP11, UHX1
Protein Information
Name USP11
Synonyms UHX1
Function Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed:12084015, PubMed:15314155, PubMed:17897950, PubMed:19874889, PubMed:20233726, PubMed:24724799, PubMed:28992046). Inhibits the degradation of target proteins by the proteasome (PubMed:12084015). Cleaves preferentially 'Lys-6' and 'Lys- 63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys- 33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed:24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed:17897950, PubMed:19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed:15314155, PubMed:20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed:20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed:28992046).
Cellular Location Nucleus. Cytoplasm. Chromosome. Note=Predominantly nuclear (PubMed:12084015, PubMed:15314155). Associates with chromatin (PubMed:20233726, PubMed:20601937).
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$ 149.00
$ 499.00
Cat# AGI1386
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