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KD-Validated Anti-CD147 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CD147 Rabbit Monoclonal Antibody AGI1420
    Western blotting analysis using anti-CD147 antibody (Cat#AGI1420). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD147 antibody (Cat#AGI1420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CD147 Rabbit Monoclonal Antibody AGI1420
    Western blotting analysis using anti-CD147 antibody (Cat#AGI1420). CD147 expression in wild type (WT) and BSG shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CD147 antibody (Cat#AGI1420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CD147 Rabbit Monoclonal Antibody AGI1420
    Flow cytometric analysis of CD147 expression in HepG2 cells using CD147 antibody (Cat#AGI1420, 1:2,000). Green, isotype control; red, CD147.
    detail
  • ICC - KD-Validated Anti-CD147 Rabbit Monoclonal Antibody AGI1420
    Immunocytochemical staining of HepG2 cells with CD147 antibody (Cat#AGI1420, 1:1,000). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P35613
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1400
Calculated MW Predicted, 42 kDa , observed, 33-65 kDa
Gene Name BSG
Aliases BSG; Basigin (Ok Blood Group); EMMPRIN; Tumor Cell-Derived Collagenase Stimulatory Factor; Extracellular Matrix Metalloproteinase Inducer; Basigin; EMPRIN; CD147; Leukocyte Activation Antigen M6; Collagenase Stimulatory Factor; Hepatoma-Associated Antigen; OK Blood Group Antigen; HAntibody18G; TCSF; 5F7; OK; Ok Blood Group; CD147 Antigen
Immunogen A synthesized peptide derived from human CD147
Additional Information
Gene ID 682
Other Names Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, EMMPRIN, Hepatoma-associated antigen, HAb18G, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF, CD147, BSG (HGNC:1116)
Protein Information
Name BSG (HGNC:1116)
Function [Isoform 1]: Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
Cellular Location Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [Isoform 2]: Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453}. Endosome Endoplasmic reticulum membrane; Single- pass type I membrane protein {ECO:0000250|UniProtKB:P26453} Basolateral cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453} [Isoform 4]: Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P26453}
Tissue Location [Isoform 1]: Retina-specific (PubMed:25957687). Expressed in retinal cone photoreceptors (at protein level) (PubMed:25957687). [Isoform 3]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus.
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$ 149.00
$ 499.00
Cat# AGI1420
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