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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody   

KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody AGI1422
    Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#AGI1422). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#AGI1422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody AGI1422
    Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#AGI1422). Phospho-vimentin (Ser72) expression in wild-type (WT) and VIM shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#AGI1422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody AGI1422
    Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using anti-Phospho-Vimentin (Ser72) antibody (Cat#AGI1422, 1:2,000). Green, isotype control; red, Phospho-Vimentin (Ser72).
    detail
  • ICC - KD-Validated Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody AGI1422
    Immunocytochemical staining of HT-1080 cells with anti-Phospho-Vimentin (Ser72) antibody (Cat#AGI1422, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P08670
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3480
Calculated MW Predicted, 54 kDa; observed, 54 kDa
Gene Name VIM
Aliases VIM; Vimentin; Epididymis Secretory Sperm Binding Protein 3
Immunogen A synthesized peptide derived from human Phospho-Vimentin (Ser72)
Additional Information
Gene ID 7431
Other Names Vimentin {ECO:0000312|HGNC:HGNC:12692}, VIM (HGNC:12692)
Protein Information
Name VIM (HGNC:12692)
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1422
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