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KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody AGI1437
    Western blotting analysis using anti-Caspase 9 antibody (Cat#AGI1437). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 9 antibody (Cat#AGI1437, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody AGI1437
    Western blotting analysis using anti-Caspase 9 antibody (Cat#AGI1437). Caspase 9 expression in wild type (WT) and Caspase 9 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Caspase 9 antibody (Cat#AGI1437, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody AGI1437
    Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#AGI1437, 1:2,000). Green, isotype control; red, Caspase 9.
    detail
  • ICC - KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody AGI1437
    Immunocytochemical staining of HepG2 cells with caspase 9 antibody (Cat#AGI1437, 1:1000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P55211
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1005
Calculated MW Predicted , 46 kDa , observed , 46 kDa
Gene Name CASP9
Aliases CASP9; Caspase 9; Apoptosis-Related Cysteine Peptidase; Regulatory Subunit 56; Apoptotic Protease Mch-6; PPP1R56; APAF-3; Apoptotic Protease Activating Factor 3; ICE-LAP6; caspase-9; MCH6; Protein Phosphatase 1; Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; CASP-9; APAF3; EC 3.4.22.62; Caspase 9; Apoptosis-Related Cysteine Protease; Apoptotic Protease-Activating Factor 3; Protein Phosphatase 1
Immunogen Recombinant protein of human Caspase-9
Additional Information
Gene ID 842
Other Names Caspase-9, CASP-9, 3.4.22.62, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, Caspase-9 subunit p35, Caspase-9 subunit p10, CASP9, MCH6
Protein Information
Name CASP9
Synonyms MCH6
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage- induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106).
Tissue Location Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1437
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