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KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody AGI1463
    Western blotting analysis using anti-Spermine synthase antibody (Cat#61824). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Spermine synthase antibody (Cat#61824, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody AGI1463
    Western blotting analysis using anti-Spermine synthase antibody (Cat#61824). Spermine synthase expression in wild type (WT) and Spermine synthase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Spermine synthase antibody (Cat#61824, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody AGI1463
    Flow cytometric analysis of Spermine synthase expression in HepG2 cells using Spermine synthase antibody (Cat#61824, 1:2,000). Green, isotype control; red, Spermine synthase
    detail
  • ICC - KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody AGI1463
    Immunocytochemical staining of HepG2 cells with Spermine synthase antibody (Cat#61824, 1:1,000). Nuclei were stained blue with DAPI; Spermine synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P52788
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4640
Calculated MW Predicted, 41 kDa , observed, 41 kDa
Gene Name SMS
Aliases SMS; Spermine Synthase; SPMSY; MRSR; Spermidine Aminopropyltransferase; SpS; SRS; Snyder-Robinson X-Linked Mental Retardation Syndrome; EC 2.5.1.22; MRXSSR; SPS
Immunogen A synthesized peptide derived from human Spermine synthase
Additional Information
Gene ID 6611
Other Names Spermine synthase, SPMSY, 2.5.1.22, Spermidine aminopropyltransferase, SMS {ECO:0000303|PubMed:14508504, ECO:0000312|HGNC:HGNC:11123}
Protein Information
Name SMS {ECO:0000303|PubMed:14508504, ECO:0000312|HGNC:HGNC:11123}
Function Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1463
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