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KD-Validated Anti-Colony Stimulating Factor 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Colony Stimulating Factor 1 Rabbit Monoclonal Antibody AGI1499
    Western blotting analysis using anti-Colony stimulating factor 1 antibody (Cat#61916). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Colony stimulating factor 1 antibody (Cat#61916, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Colony Stimulating Factor 1 Rabbit Monoclonal Antibody AGI1499
    Western blotting analysis using anti-Colony stimulating factor 1 antibody (Cat#61916). Colony stimulating factor 1 expression in wild type (WT) and Colony stimulating factor 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Colony stimulating factor 1 antibody (Cat.#61916, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Colony Stimulating Factor 1 Rabbit Monoclonal Antibody AGI1499
    Flow cytometric analysis of Colony stimulating factor 1 expression in HAP-1 cells using Colony stimulating factor 1 antibody (Cat#61916, 1:2,000). Green, isotype control; red, Colony stimulating factor 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P09603
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5110
Calculated MW Predicted, 60 kDa, observed, 30 kDa
Gene Name CSF1
Aliases CSF1; Colony Stimulating Factor 1; MCSF; M-CSF; Proteoglycan Macrophage Colony-Stimulating Factor; Colony Stimulating Factor 1 (Macrophage); Macrophage Colony Stimulating Factor 1; Macrophage Colony-Stimulating Factor 1; Lanimostim; MGC31930; PG-M-CSF; CSF-1; CSF1-HERV-LTR71B Fusion Protein
Immunogen A synthesized peptide derived from human MCSF
Additional Information
Gene ID 1435
Other Names Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, CSF1
Protein Information
Name CSF1
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1499
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