Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Diacylglycerol O-acyltransferase 1 Rabbit Monoclonal Antibody   

KD-Validated Anti-Diacylglycerol O-acyltransferase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Diacylglycerol O-acyltransferase 1 Rabbit Monoclonal Antibody AGI1503
    Western blotting analysis using anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#AGI1503). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#AGI1503, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Diacylglycerol O-acyltransferase 1 Rabbit Monoclonal Antibody AGI1503
    Western blotting analysis using anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#AGI1503). Diacylglycerol O-acyltransferase 1 expression in wild type (WT) and Diacylglycerol O-acyltransferase 1 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#AGI1503, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Diacylglycerol O-acyltransferase 1 Rabbit Monoclonal Antibody AGI1503
    Flow cytometric analysis of Diacylglycerol O-acyltransferase 1 expression in HeLa cells using Diacylglycerol O-acyltransferase 1 antibody (Cat#AGI1503, 1:2,000). Green, isotype control; red, Diacylglycerol O-acyltransferase 1.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O75907
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5155
Calculated MW Predicted, 55 kDa , observed, 48 kDa
Gene Name DGAT1
Aliases DGAT1; Diacylglycerol O-Acyltransferase 1; ARGP1; DGAT; Acyl-CoA Retinol O-Fatty-Acyltransferase; Diglyceride Acyltransferase; EC 2.3.1.20; ARAT; Acyl Coenzyme A:Cholesterol Acyltransferase Related Gene 1; Diacylglycerol O-Acyltransferase (Mouse) Homolog; Acyl-CoA:Diacylglycerol Acyltransferase; Retinol O-Fatty-Acyltransferase; ACAT Related Gene Product 1; ACAT-Related Gene Product 1; EC 2.3.1.76; EC 2.3.1; DIAR7; AGRP1
Immunogen A synthesized peptide derived from human DGAT1
Additional Information
Gene ID 8694
Other Names Diacylglycerol O-acyltransferase 1, 2.3.1.20, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, 2.3.1.76, Diglyceride acyltransferase, DGAT1 {ECO:0000303|PubMed:16214399, ECO:0000312|HGNC:HGNC:2843}
Protein Information
Name DGAT1 {ECO:0000303|PubMed:16214399, ECO:0000312|HGNC:HGNC:2843}
Function Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:16214399, PubMed:18768481, PubMed:28420705, PubMed:32433610, PubMed:32433611, PubMed:9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed:18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed:18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Z2A7}; Multi-pass membrane protein
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 149.00
$ 499.00
Cat# AGI1503
Size:
Quantity:
Availability:
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaMexicoNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"