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KD-Validated Anti-Fatty acid synthase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Fatty acid synthase Rabbit Monoclonal Antibody AGI1520
    Western blotting analysis using anti-Fatty acid synthase antibody (Cat#61976). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fatty acid synthase antibody (Cat#61976, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Fatty acid synthase Rabbit Monoclonal Antibody AGI1520
    Western blotting analysis using anti-Fatty acid synthase antibody (Cat#61976). Fatty acid synthase expression in wild type (WT) and Fatty acid synthase(FASN) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Fatty acid synthase antibody (Cat#61976, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Fatty acid synthase Rabbit Monoclonal Antibody AGI1520
    Flow cytometric analysis of Fatty acid synthase expression in HepG2 cells using Fatty acid synthase antibody (Cat#61976, 1:2,000). Green, isotype control; red, Fatty acid synthase.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P49327
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5380
Calculated MW Predicted, 273 kDa, observed,273 kDa
Gene Name FASN
Aliases FASN; Fatty Acid Synthase; FAS; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X, Member 1; 3-Hydroxyacyl-[Acyl-Carrier-Protein] Dehydratase; [Acyl-Carrier-Protein] S-Malonyltransferase; [Acyl-Carrier-Protein] S-Acetyltransferase; 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; 3-Oxoacyl-[Acyl-Carrier-Protein] Synthase; Enoyl-[Acyl-Carrier-Protein] Reductase; Acyl-[Acyl-Carrier-Protein] Hydrolase; Type I Fatty Acid Synthase; EC 2.3.1.85; EC 6.3.3.1; EC 2.3.1; OA-519
Immunogen A synthesized peptide derived from human FASN
Additional Information
Gene ID 2194
Other Names Fatty acid synthase, 2.3.1.85, Type I fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, 2.3.1.38, [Acyl-carrier-protein] S-malonyltransferase, 2.3.1.39, 3-oxoacyl-[acyl-carrier-protein] synthase, 2.3.1.41, 3-oxoacyl-[acyl-carrier-protein] reductase, 1.1.1.100, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 4.2.1.59, Enoyl-[acyl-carrier-protein] reductase, 1.3.1.39, Acyl-[acyl-carrier-protein] hydrolase, 3.1.2.14, FASN, FAS
Protein Information
Name FASN
Synonyms FAS
Function Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Cellular Location Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1520
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