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KD-Validated Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody AGI1526
    Western blotting analysis using anti-Golgi integral membrane protein 4 antibody (Cat#AGI1526). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Golgi integral membrane protein 4 antibody (Cat#AGI1526, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody AGI1526
    Western blotting analysis using anti-Golgi integral membrane protein 4 antibody (Cat#AGI1526). Golgi integral membrane protein 4 expression in wild type (WT) and Golgi integral membrane protein 4 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Golgi integral membrane protein 4 antibody (Cat#AGI1526, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody AGI1526
    Flow cytometric analysis of Golgi integral membrane protein 4 expression in HAP-1 cells using Golgi integral membrane protein 4 antibody (Cat#AGI1526, 1:2,000). Green, isotype control; red, Golgi integral membrane protein 4.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O00461
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5575
Calculated MW Predicted, 82 kDa , observed, 130 kDa
Gene Name GOLIM4
Aliases GOLIM4; Golgi Integral Membrane Protein 4; GPP130; GIMPC; Golgi Phosphoprotein 4; GOLPH4; P138; Golgi-Localized Phosphoprotein Of 130 KDa; Golgi Integral Membrane Protein, Cis; Golgi Phosphoprotein Of 130 KDa; Cis Golgi-Localized Calcium-Binding Protein; 130 KDa Golgi-Localized Phosphoprotein; Type II Golgi Membrane Protein; GIMPc
Immunogen A synthesized peptide derived from human GOLPH4
Additional Information
Gene ID 27333
Other Names Golgi integral membrane protein 4, Golgi integral membrane protein, cis, GIMPc, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, Golgi phosphoprotein of 130 kDa, GOLIM4, GIMPC, GOLPH4, GPP130
Protein Information
Name GOLIM4
Synonyms GIMPC, GOLPH4, GPP130
Function Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Cellular Location Golgi apparatus, Golgi stack membrane; Single- pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein. Membrane; Lipid-anchor Note=Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1526
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