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KD-Validated Anti-KHSRP Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-KHSRP Rabbit Monoclonal Antibody AGI1566
    Western blotting analysis using anti-KHSRP antibody (Cat#62192). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KHSRP antibody (Cat#62192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-KHSRP Rabbit Monoclonal Antibody AGI1566
    Western blotting analysis using anti-KHSRP antibody (Cat#62192). KHSRP expression in wild type (WT) and KHSRP shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-KHSRP antibody (Cat#62192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-KHSRP Rabbit Monoclonal Antibody AGI1566
    Flow cytometric analysis of KHSRP expression in HepG2 cells using KHSRP antibody (Cat#62192, 1:2,000). Green, isotype control; red, KHSRP.
    detail
  • ICC - KD-Validated Anti-KHSRP Rabbit Monoclonal Antibody AGI1566
    Immunocytochemical staining of HepG2 cells with anti-KHSRP antibody (Cat #62192, 1:1,000). Nuclei were stained blue with DAPI; KHSRP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q92945
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB6435
Calculated MW Predicted, 73 kDa , observed , 73 kDa
Gene Name KHSRP
Aliases KHSRP; KH-Type Splicing Regulatory Protein; FUBP2; KSRP; FBP2; Far Upstream Element-Binding Protein; FUSE-Binding Protein; P75; Epididymis Secretory Sperm Binding Protein; KH Type-Splicing Regulatory Protein; FUSE Binding Protein 2
Immunogen A synthesized peptide derived from human KHSRP
Additional Information
Gene ID 8570
Other Names Far upstream element-binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, KSRP, p75, KHSRP, FUBP2
Protein Information
Name KHSRP
Synonyms FUBP2
Function Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue- specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Cellular Location Nucleus. Cytoplasm. Note=A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
Tissue Location Detected in neural and non-neural cell lines.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1566
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