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KD-Validated Anti-Cyclin dependent kinase 16 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin dependent kinase 16 Rabbit Monoclonal Antibody AGI1577
    Western blotting analysis using anti-Cyclin dependent kinase 16 antibody (Cat#AGI1577). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase 16 antibody (Cat#AGI1577, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Cyclin dependent kinase 16 Rabbit Monoclonal Antibody AGI1577
    Western blotting analysis using anti-Cyclin dependent kinase 16 antibody (Cat#AGI1577). Cyclin dependent kinase 16 expression in wild type (WT) and cyclin dependent kinase 16 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin dependent kinase 16 antibody (Cat#AGI1577, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Cyclin dependent kinase 16 Rabbit Monoclonal Antibody AGI1577
    Flow cytometric analysis of Cyclin dependent kinase 16 expression in HepG2 cells using Cyclin dependent kinase 16 antibody (Cat#AGI1577, 1:2,000). Green, isotype control; red, Cyclin dependent kinase 16.
    detail
  • ICC - KD-Validated Anti-Cyclin dependent kinase 16 Rabbit Monoclonal Antibody AGI1577
    Immunocytochemical staining of HepG2 cells with Cyclin dependent kinase 16 antibody (AGI1577, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase 16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q00536
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1495
Calculated MW Predicted, 56 kDa , observed, 62-68 kDa
Gene Name CDK16
Aliases CDK16; Cyclin Dependent Kinase 16; PCTAIRE1; PCTGAIRE; PCTAIRE; PCTK1; Serine/Threonine-Protein; Kinase PCTAIRE-1; Cell Division Protein Kinase 16; PCTAIRE-Motif Protein Kinase; Cyclin-Dependent Kinase 16; EC 2.7.11.22; FLJ16665; Testis Secretory Sperm-Binding Protein Li 224n; Serine/Threonine-Protein Kinase; PCTAIRE Protein Kinase; EC 2.7.11
Immunogen A synthesized peptide derived from human PCTAIRE1
Additional Information
Gene ID 5127
Other Names Cyclin-dependent kinase 16, 2.7.11.22, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1, CDK16, PCTAIRE1, PCTK1
Protein Information
Name CDK16
Synonyms PCTAIRE1, PCTK1
Function Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Cellular Location Cytoplasm. Cytoplasmic vesicle, secretory vesicle {ECO:0000250|UniProtKB:Q63686} Cell membrane; Peripheral membrane protein; Cytoplasmic side. Synapse, synaptosome {ECO:0000250|UniProtKB:Q63686}. Note=Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY
Tissue Location Detected in pancreas islets (at protein level). Detected in brain and pancreas.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1577
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