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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Glutamic-oxaloacetic transaminase 2 Rabbit Monoclonal Antibody   

KD-Validated Anti-Glutamic-oxaloacetic transaminase 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Glutamic-oxaloacetic transaminase 2 Rabbit Monoclonal Antibody AGI1579
    Western blotting analysis using anti-Glutamic-oxaloacetic transaminase 2 antibody (Cat#AGI1579). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamic-oxaloacetic transaminase 2 antibody (Cat#AGI1579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Glutamic-oxaloacetic transaminase 2 Rabbit Monoclonal Antibody AGI1579
    Western blotting analysis using anti-Glutamic-oxaloacetic transaminase 2 antibody (Cat#AGI1579). Glutamic-oxaloacetic transaminase 2 expression in wild type (WT) and Glutamic-oxaloacetic transaminase 2 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Glutamic-oxaloacetic transaminase 2 antibody (Cat#AGI1579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Glutamic-oxaloacetic transaminase 2 Rabbit Monoclonal Antibody AGI1579
    Flow cytometric analysis of Glutamic-oxaloacetic transaminase 2 expression in HepG2 cells using Glutamic-oxaloacetic transaminase 2 antibody (Cat#AGI1579, 1:2,000). Green, isotype control; red, Glutamic-oxaloacetic transaminase 2.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P00505
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB7985
Calculated MW Predicted, 48 kDa , observed , 43 kDa
Gene Name GOT2
Aliases GOT2; Glutamic-Oxaloacetic Transaminase 2; KYAT4; Kynurenine Aminotransferase IV; MAspAT; KATIV; KAT4; Plasma Membrane-Associated Fatty Acid-Binding Protein; Glutamic-Oxaloacetic Transaminase 2, Mitochondrial; Aspartate Aminotransferase, Mitochondrial; Kynurenine--Oxoglutarate Transaminase IV; Kynurenine--Oxoglutarate Transaminase 4; Glutamate Oxaloacetate Transaminase 2; Kynurenine Aminotransferase 4; Aspartate Aminotransferase 2; Fatty Acid-Binding Protein; Aspartate Transaminase 2; Transaminase A; EC 2.6.1.1; MitAAT; FABP-1; FABPpm; Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2); EC 2.6.1.7; EC 2.6.1; MITAAT; DEE82
Immunogen A synthesized peptide derived from human GOT2
Additional Information
Gene ID 2806
Other Names Aspartate aminotransferase, mitochondrial, mAspAT, 2.6.1.1, 2.6.1.7, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, GOT2 (HGNC:4433)
Protein Information
Name GOT2 (HGNC:4433)
Function Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Cellular Location Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1579
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