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KD-Validated Anti-LPP Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-LPP Rabbit Monoclonal Antibody AGI1581
    Western blotting analysis using anti-LPP antibody (Cat#AGI1581). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LPP antibody (Cat#AGI1581, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-LPP Rabbit Monoclonal Antibody AGI1581
    Western blotting analysis using anti-LPP antibody (Cat#AGI1581). LPP expression in wild type (WT) and LPP shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LPP antibody (Cat#AGI1581, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-LPP Rabbit Monoclonal Antibody AGI1581
    Flow cytometric analysis of LPP expression in HT-1080 cells using anti-LPP antibody (Cat#AGI1581, 1:2,000). Green, isotype control; red, LPP.
    detail
  • ICC - KD-Validated Anti-LPP Rabbit Monoclonal Antibody AGI1581
    Immunocytochemical staining of HT-1080 cells with anti-LPP antibody (Cat#AGI1581, 1:1,000). Nuclei were stained blue with DAPI; LPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q93052
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB05
Calculated MW Predicted, 66 kDa , observed , 70 kDa
Gene Name LPP
Aliases LIM Domain Containing Preferred Translocation Partner In Lipoma; LIM Domain-Containing Preferred Translocation Partner In Lipoma; Lipoma-Preferred Partner; Lipoma Preferred Partner Gene; Lipoma Preferred Partner; LIM Protein
Immunogen A synthesized peptide derived from human LPP
Additional Information
Gene ID 4026
Other Names Lipoma-preferred partner, LIM domain-containing preferred translocation partner in lipoma, LPP
Protein Information
Name LPP
Function May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
Cellular Location Nucleus. Cytoplasm. Cell junction. Cell membrane. Note=Found in the nucleus, in the cytoplasm and at cell adhesion sites Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and KMT2A/MLL1
Tissue Location Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1581
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