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KD-Validated Anti-NDUFB9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-NDUFB9 Rabbit Monoclonal Antibody AGI1586
    Western blotting analysis using anti-NDUFB9 antibody (Cat#62276). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB9 antibody (Cat#62276, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-NDUFB9 Rabbit Monoclonal Antibody AGI1586
    Western blotting analysis using anti-NDUFB9 antibody (Cat#62276). NDUFB9 expression in wild type (WT) and NDUFB9 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NDUFB9 antibody (Cat#62276, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-NDUFB9 Rabbit Monoclonal Antibody AGI1586
    Flow cytometric analysis of NDUFB9 expression in C2C12 cells using NDUFB9 antibody (Cat#62276, 1:2,000). Green, isotype control; red, NDUFB9.
    detail
  • ICC - KD-Validated Anti-NDUFB9 Rabbit Monoclonal Antibody AGI1586
    Immunocytochemical staining of C2C12 cells with anti-NDUFB9 antibody (Cat#62276, 1:1,000). Nuclei were stained blue with DAPI; NDUFB9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9Y6M9
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB90
Calculated MW Predicted, 22 kDa , observed , 22 kDa
Gene Name NDUFB9
Aliases NADH:Ubiquinone Oxidoreductase Subunit B9; UQOR22; LYRM3; B22; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 9, 22kDa; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 9; NADH-Ubiquinone Oxidoreductase B22 Subunit; LYR Motif-Containing Protein 3; Complex I B22 Subunit; CI-B22; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 9 (22kD, B22); Complex I-B22; MC1DN24
Immunogen A synthesized peptide derived from human NDUFB9
Additional Information
Gene ID 4715
Other Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, Complex I-B22, CI-B22, LYR motif-containing protein 3, NADH-ubiquinone oxidoreductase B22 subunit, NDUFB9, LYRM3, UQOR22
Protein Information
Name NDUFB9
Synonyms LYRM3, UQOR22
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1586
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