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KD-Validated Anti-NQO2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-NQO2 Rabbit Monoclonal Antibody AGI1592
    Western blotting analysis using anti-NQO2 antibody (Cat#62309). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NQO2 antibody (Cat#62309, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-NQO2 Rabbit Monoclonal Antibody AGI1592
    Western blotting analysis using anti-NQO2 antibody (Cat#62309). NQO2 expression in wild type (WT) and NQO2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NQO2 antibody (Cat#62309, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • ICC - KD-Validated Anti-NQO2 Rabbit Monoclonal Antibody AGI1592
    Immunocytochemical staining of HepG2 cells with anti-NQO2 antibody (Cat#62309, 1:1,000). Nuclei were stained blue with DAPI; NQO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P16083
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB250
Calculated MW Predicted, 26 kDa , observed , 26 kDa
Gene Name NQO2
Aliases NQO2; N-Ribosyldihydronicotinamide:Quinone Dehydrogenase 2; QR2; N-Ribosyldihydronicotinamide:Quinone Reductase 2; NRH:Quinone Oxidoreductase 2; Quinone Reductase 2; NMOR2; DHQV; DIA6; Ribosyldihydronicotinamide Dehydrogenase [Quinone]; NAD(P)H Quinone Dehydrogenase 2; NAD(P)H Menadione Oxidoreductase-1, Dioxin-Inducible-2; NAD(P)H Menadione Oxidoreductase 2, Dioxin-Inducible; Ribosyldihydronicotinamide Dehydrogenase; NAD(P)H Dehydrogenase, Quinone 2; NRH Dehydrogenase [Quinone] 2; EC 1.10.5.1
Immunogen A synthesized peptide derived from human NQO2
Additional Information
Gene ID 4835
Other Names Ribosyldihydronicotinamide dehydrogenase [quinone], 1.10.5.1, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2, NQO2, NMOR2
Protein Information
Name NQO2
Synonyms NMOR2
Function The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1592
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