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KD-Validated Anti-Nemo Like Kinase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Nemo Like Kinase Rabbit Monoclonal Antibody AGI1616
    Western blotting analysis using anti-Nemo like kinase antibody (Cat#AGI1616). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nemo like kinase antibody (Cat#AGI1616, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Nemo Like Kinase Rabbit Monoclonal Antibody AGI1616
    Western blotting analysis using anti-Nemo like kinase antibody (Cat#AGI1616). Nemo like kinase expression in wild type (WT) and Nemo like kinase shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Nemo like kinase antibody (Cat#AGI1616, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Nemo Like Kinase Rabbit Monoclonal Antibody AGI1616
    Flow cytometric analysis of Nemo like kinase expression in HeLa cells using Nemo like kinase antibody (Cat#AGI1616, 1:2,000). Green, isotype control; red, Nemo like kinase.
    detail
  • ICC - KD-Validated Anti-Nemo Like Kinase Rabbit Monoclonal Antibody AGI1616
    Immunocytochemical staining of HeLa cells with anti-Nemo like kinase antibody (Cat#AGI1616, 1:1,000). Nuclei were stained blue with DAPI; Nemo like kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9UBE8
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB585
Calculated MW Predicted, 58 kDa ; Observed , 58 kDa
Gene Name NLK
Aliases Nemo Like Kinase; Serine/Threonine-Protein Kinase NLK; Nemo-Like Kinase; EC 2.7.11.24; Protein LAK1; EC 2.7.11; LAK1
Immunogen A synthesized peptide derived from human NLK
Additional Information
Gene ID 51701
Other Names Serine/threonine-protein kinase NLK, 2.7.11.24, Nemo-like kinase, Protein LAK1, NLK, LAK1 {ECO:0000312|EMBL:AAD56013.1}
Protein Information
Name NLK
Synonyms LAK1 {ECO:0000312|EMBL:AAD56013.1}
Function Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed:12482967, PubMed:14960582, PubMed:15004007, PubMed:15764709, PubMed:20061393, PubMed:20874444, PubMed:21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed:15004007, PubMed:15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed:12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed:21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed:12482967, PubMed:21454679). Negative regulator of the Notch signaling pathway (PubMed:20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed:20118921). Negative regulator of the MYB family of transcription factors (PubMed:15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed:15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed:15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed:15004007, PubMed:15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed:26588989).
Cellular Location Nucleus {ECO:0000250|UniProtKB:O54949}. Cytoplasm {ECO:0000250|UniProtKB:O54949}. Note=Predominantly nuclear. A smaller fraction is cytoplasmic. {ECO:0000250|UniProtKB:O54949}
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$ 149.00
$ 499.00
Cat# AGI1616
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