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KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody AGI1625
    Western blotting analysis using anti-PCBD1 antibody (Cat#62427). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody AGI1625
    Western blotting analysis using anti-PCBD1 antibody (Cat#62427). PCBD1 expression in wild-type (WT) and PCBD1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PCBD1 antibody (Cat#62427, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • ICC - KD-Validated Anti-PCBD1 Rabbit Monoclonal Antibody AGI1625
    Immunocytochemical staining of HepG2 cells with anti-PCBD1 antibody (Cat#62427, 1:1,000). Nuclei were stained blue with DAPI; PCBD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P61457
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB785
Calculated MW Predicted, 12 kDa , observed , 12 kDa
Gene Name PCBD1
Aliases Pterin-4 Alpha-Carbinolamine Dehydratase 1; PCD; DCOH; PCBD; 6-Pyruvoyl-Tetrahydropterin Synthase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4 Alpha-Carbinolamine Dehydratase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha; Phenylalanine Hydroxylase-Stimulating Protein; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Pterin-4-Alpha-Carbinolamine Dehydratase; Dimerizing Cofactor For HNF1; EC 4.2.1.96; PHS; Pterin-4 Alpha-Carbinolamine Dehydratase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4a-Carbinolamine Dehydratase (Dimerization Cofactor Of Hepatic Nuclear Factor 1-Alpha); Dimerization Cofactor Of Hepatocyte Nuclear Factor 1-Alpha; Pterin-4-Alpha Carbinolamine Dehydratase; Pterin Carbinolamine Dehydratase; Dimerization Cofactor Of HNF1; DCoH
Immunogen A synthesized peptide derived from human PCBD1
Additional Information
Gene ID 5092
Other Names Pterin-4-alpha-carbinolamine dehydratase, PHS, 4.2.1.96, 4-alpha-hydroxy-tetrahydropterin dehydratase, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCoH, Dimerization cofactor of HNF1, Phenylalanine hydroxylase-stimulating protein, Pterin carbinolamine dehydratase, PCD, PCBD1, DCOH, PCBD
Protein Information
Name PCBD1
Synonyms DCOH, PCBD
Function Involved in tetrahydrobiopterin biosynthesis (By similarity). Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription (By similarity). Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity (By similarity). Also acts as a coactivator for HNF1B-dependent transcription (PubMed:24204001).
Cellular Location Cytoplasm. Nucleus. Note=Recruited to the nucleus through the interaction with HNF1B.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1625
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