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KD-Validated Anti-PEF1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PEF1 Rabbit Monoclonal Antibody AGI1674
    Western blotting analysis using anti-PEF1 antibody (Cat#62544). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEF1 antibody (Cat#62544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-PEF1 Rabbit Monoclonal Antibody AGI1674
    Western blotting analysis using anti-PEF1 antibody (Cat#62544). PEF1 expression in wild type (WT) and PEF1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PEF1 antibody (Cat#62544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-PEF1 Rabbit Monoclonal Antibody AGI1674
    Flow cytometric analysis of PEF1 expression in Hela cells using anti-PEF1 antibody (Cat#62544, 1:2,000). Green, isotype control; red, PEF1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q9UBV8
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1340
Calculated MW Predicted, 30 kDa , observed, 30 kDa
Gene Name PEF1
Aliases PEF1; Penta-EF-Hand Domain Containing 1; Peflin; PEF1A; PEF Protein With A Long N-Terminal Hydrophobic Domain; ABP32; Epididymis Secretory Sperm Binding Protein; Penta-EF Hand Domain-Containing Protein 1
Immunogen A synthesized peptide derived from human PEF1
Additional Information
Gene ID 553115
Other Names Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1 {ECO:0000312|HGNC:HGNC:30009}, PEF1 (HGNC:30009), ABP32
Protein Information
Name PEF1 (HGNC:30009)
Synonyms ABP32
Function Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
Cellular Location Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q641Z8}. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+) (PubMed:11278427). Localizes to endoplasmic reticulum exit site (ERES) (By similarity). {ECO:0000250|UniProtKB:Q641Z8, ECO:0000269|PubMed:11278427}
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$ 149.00
$ 499.00
Cat# AGI1674
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