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KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody AGI1678
    Western blotting analysis using anti-RAD21 antibody (Cat#AGI1678). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD21 antibody (Cat#AGI1678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody AGI1678
    Western blotting analysis using anti-RAD21 antibody (Cat#AGI1678). RAD21 expression in wild type (WT) and RAD21 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAD21 antibody (Cat#AGI1678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody AGI1678
    Flow cytometric analysis of RAD21 expression in HepG2 cells using anti-RAD21 antibody (Cat#AGI1678, 1:2,000). Green, isotype control; red, RAD21.
    detail
  • ICC - KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody AGI1678
    Immunocytochemical staining of HepG2 cells with anti-RAD21 antibody (Cat#AGI1678, 1:1,000). Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O60216
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1390
Calculated MW Predicted, 72 kDa , observed, 130 kDa
Gene Name RAD21
Aliases RAD21; RAD21 Cohesin Complex Component; HHR21; SCC1; KIAA0078; Double-Strand-Break Repair Protein Rad21 Homolog; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair; RAD21 (S. Pombe) Homolog; RAD21 Homolog (S. Pombe); RAD21 Homolog; HRAD21; CDLS4; MCD1; MGS
Immunogen A synthesized peptide derived from human RAD21
Additional Information
Gene ID 5885
Other Names Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, 64-kDa C-terminal product, 64-kDa carboxy-terminal product, 65-kDa carboxy-terminal product, RAD21
Protein Information
Name RAD21
Function [Double-strand-break repair protein rad21 homolog]: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).
Cellular Location [Double-strand-break repair protein rad21 homolog]: Nucleus. Nucleus matrix Chromosome Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=Associates with chromatin (PubMed:11073952, PubMed:11590136). Before prophase, scattered along chromosome arms (PubMed:11073952). During prophase and prometaphase, most cohesins dissociate from the arms of condensing chromosome, possibly through PLK1-mediated phosphorylation (PubMed:11931760). A small amount of cohesin remains in centromeric regions and is removed from chromosomes only at the onset of anaphase. At anaphase, cleavage by separase/ESPL1 leads to the dissociation of cohesin from chromosomes and chromosome separation (PubMed:11073952, PubMed:11509732)
Tissue Location Expressed in the gut (at protein level).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1678
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