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KD-Validated Anti-Radixin Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Radixin Rabbit Monoclonal Antibody AGI1680
    Western blotting analysis using anti-Radixin antibody (Cat#AGI1680). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Radixin antibody (Cat#AGI1680, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Radixin Rabbit Monoclonal Antibody AGI1680
    Western blotting analysis using anti-Radixin antibody (Cat#AGI1680). Radixin expression in wild type (WT) and Radixin shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Radixin antibody (Cat#AGI1680, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Radixin Rabbit Monoclonal Antibody AGI1680
    Flow cytometric analysis of Radixin expression in HepG2 cells using anti-Radixin antibody (Cat#AGI1680, 1:2,000). Green, isotype control; red, Radixin.
    detail
  • ICC - KD-Validated Anti-Radixin Rabbit Monoclonal Antibody AGI1680
    Immunocytochemical staining of HepG2 cells with anti-Radixin antibody (Cat#AGI1680, 1:1,000). Nuclei were stained blue with DAPI; Radixin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P35241
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1420
Calculated MW Predicted, 69 kDa , observed, 75 kDa
Gene Name RDX
Aliases RDX; Radixin; DFNB24; Deafness, Autosomal Recessive 24
Immunogen A synthesized peptide derived from human Radixin
Additional Information
Gene ID 5962
Other Names Radixin, RDX
Protein Information
Name RDX
Function Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cell projection, microvillus {ECO:0000250|UniProtKB:P26043}. Cell projection, stereocilium {ECO:0000250|UniProtKB:P26043}. Note=Enriched at the stereocilium base with very low levels in the shaft of stereociliary bundles (By similarity). Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively {ECO:0000250|UniProtKB:P26043}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1680
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