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KD-Validated Anti-Ubiquitin Specific Peptidase 14 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Ubiquitin Specific Peptidase 14 Rabbit Monoclonal Antibody AGI1710
    Western blotting analysis using anti-ubiquitin specific peptidase 14 antibody (Cat#62631). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitin specific peptidase 14 antibody (Cat#62631, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Ubiquitin Specific Peptidase 14 Rabbit Monoclonal Antibody AGI1710
    Western blotting analysis using anti-Ubiquitin specific peptidase 14 antibody (Cat#62631). Ubiquitin specific peptidase 14 expression in wild type (WT) and Ubiquitin specific peptidase 14 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Ubiquitin Specific Peptidase 14 Rabbit Monoclonal Antibody AGI1710
    Flow cytometric analysis of Ubiquitin specific peptidase 14 expression in HepG2 cells using anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:2,000). Green, isotype control; red, Ubiquitin specific peptidase 14.
    detail
  • ICC - KD-Validated Anti-Ubiquitin Specific Peptidase 14 Rabbit Monoclonal Antibody AGI1710
    Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P54578
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2190
Calculated MW Predicted, 56 kDa , observed , 56 kDa
Gene Name USP14
Aliases USP14; Ubiquitin Specific Peptidase 14; TGT; Ubp6; Ubiquitin Specific Peptidase 14 (TRNA-Guanine Transglycosylase); Ubiquitin Specific Protease 14 (TRNA-Guanine Transglycosylase); Ubiquitin Carboxyl-Terminal Hydrolase 14; Deubiquitinating Enzyme 14; Ubiquitin Thioesterase 14; TRNA-Guanine Transglycosylase, 60-KD Subunit; Ubiquitin-Specific Processing Protease 14; Ubiquitin-Specific-Processing Protease 14; Ubiquitin Thiolesterase 14; EC 3.4.19.12; EC 3.1.2.15
Immunogen A synthesized peptide derived from human USP14
Additional Information
Gene ID 9097
Other Names Ubiquitin carboxyl-terminal hydrolase 14, 3.4.19.12, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14, USP14, TGT
Protein Information
Name USP14
Synonyms TGT
Function Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1710
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