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KD-Validated Anti-Serine Racemase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Serine Racemase Rabbit Monoclonal Antibody AGI1728
    Western blotting analysis using anti-Serine racemase antibody (Cat#AGI1728). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Serine racemase antibody (Cat#AGI1728, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Serine Racemase Rabbit Monoclonal Antibody AGI1728
    Western blotting analysis using anti-Serine racemase antibody (Cat#AGI1728). Serine racemase expression in wild type (WT) and Serine racemase shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Serine racemase antibody (Cat#AGI1728, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Serine Racemase Rabbit Monoclonal Antibody AGI1728
    Flow cytometric analysis of Serine racemase expression in HepG2 cells using anti-Serine racemase antibody (Cat#AGI1728, 1:2,000). Green, isotype control; red, Serine racemase.
    detail
  • ICC - KD-Validated Anti-Serine Racemase Rabbit Monoclonal Antibody AGI1728
    Immunocytochemical staining of HepG2 cells with anti-Serine racemase antibody (Cat#AGI1728, 1:1,000). Nuclei were stained blue with DAPI; Serine racemase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9GZT4
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2345
Calculated MW Predicted, 37 kDa , observed , 34-36 kDa
Gene Name SRR
Aliases SRR; Serine Racemase; ILV1; ISO1; D-Serine Ammonia-Lyase; L-Serine Ammonia-Lyase; D-Serine Dehydratase; L-Serine Dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17
Immunogen A synthesized peptide derived from human Serine racemase
Additional Information
Gene ID 63826
Other Names Serine racemase, 5.1.1.18, D-serine ammonia-lyase, D-serine dehydratase, 4.3.1.18, SRR
Protein Information
Name SRR
Function Catalyzes the synthesis of D-serine from L-serine (PubMed:20106978, PubMed:23391306, PubMed:29277459). D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine (By similarity).
Tissue Location Expressed in the cerebellum, hippocampus, dorsolateral prefrontal cortex, and in motor neurons and glial cells of the lumbar spinal cord (at protein level) (PubMed:17880399, PubMed:24138986). Increased in the dorsolateral prefrontal cortex of schizophrenic patients (at protein level) (PubMed:17880399). Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei (PubMed:15193426). Expressed in heart, skeletal muscle, kidney, and liver (PubMed:15193426)
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1728
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