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KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody AGI1730
    Western blotting analysis using anti-U2AF2 antibody (Cat#AGI1730). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-U2AF2 antibody (Cat#AGI1730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody AGI1730
    Western blotting analysis using anti-U2AF2 antibody (Cat#AGI1730). U2AF2 expression in wild-type (WT) and U2AF2 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-U2AF2 antibody (Cat#AGI1730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody AGI1730
    Flow cytometric analysis of U2AF2 expression in HepG2 cells using anti-U2AF2 antibody (Cat#AGI1730, 1:2,000). Green, isotype control; red, U2AF2.
    detail
  • ICC - KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody AGI1730
    Immunocytochemical staining of HepG2 cells with anti-U2AF2 antibody (Cat#AGI1730, 1:1,000). Nuclei were stained blue with DAPI; U2AF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P26368
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2465
Calculated MW Predicted, 54 kDa , observed , 54 kDa
Gene Name U2AF2
Aliases U2AF2; U2 Small Nuclear RNA Auxiliary Factor 2; U2AF65; U2 SnRNP Auxiliary Factor Large Subunit; U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD); U2 (RNU2) Small Nuclear RNA Auxiliary Factor 2; Splicing Factor U2AF 65 KDa Subunit; U2 Auxiliary Factor 65 KDa Subunit; HU2AF65; Splicing Factor U2AF 65 KD Subunit; HU2AF(65); DEVDFB
Immunogen A synthesized peptide derived from human U2AF65
Additional Information
Gene ID 11338
Other Names Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit, U2AF2, U2AF65
Protein Information
Name U2AF2
Synonyms U2AF65
Function Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186). By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).
Cellular Location Nucleus.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1730
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