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KD-Validated Anti-Versican Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Versican Rabbit Monoclonal Antibody AGI1743
    Western blotting analysis using anti-Versican antibody (Cat#AGI1743). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Versican antibody (Cat#AGI1743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Versican Rabbit Monoclonal Antibody AGI1743
    Western blotting analysis using anti-versican antibody (Cat#AGI1743). Versican expression in wild-type (WT) and versican (VCAN) shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-versican antibody (Cat#AGI1743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Versican Rabbit Monoclonal Antibody AGI1743
    Flow cytometric analysis of Versican expression in HepG2 cells using anti-Versican antibody (Cat#AGI1743, 1:2,000). Green, isotype control; red, Versican.
    detail
  • ICC - KD-Validated Anti-Versican Rabbit Monoclonal Antibody AGI1743
    Immunocytochemical staining of HepG2 cells with anti-Versican antibody (Cat#AGI1743, 1:1,000). Nuclei were stained blue with DAPI; Versican was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P13611
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2380
Calculated MW Predicted, 373 kDa , observed , 373 kDa
Gene Name VCAN
Aliases VCAN; Versican; PG-M; CSPG2; Chondroitin Sulfate Proteoglycan Core Protein; Chondroitin Sulfate Proteoglycan 2; Glial Hyaluronate-Binding Protein; Large Fibroblast Proteoglycan; Versican Proteoglycan; Versican Core Protein; GHAP; ERVR; WGN1; WGN
Immunogen A synthesized peptide derived from human Versican
Additional Information
Gene ID 1462
Other Names Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M, VCAN, CSPG2
Protein Information
Name VCAN
Synonyms CSPG2
Function May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Cellular Location Secreted, extracellular space, extracellular matrix. Cell projection, cilium, photoreceptor outer segment. Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix. Secreted
Tissue Location Detected in placenta (at protein level) (PubMed:32337544). Detected in cerebrospinal fluid, fibroblasts and urine (at protein level) (PubMed:25326458, PubMed:36213313). Expressed in the retina (at protein level) (PubMed:29777959). Cerebral white matter and plasma (PubMed:2469524). Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V2: Restricted to normal brain and gliomas (PubMed:8627343). Isoform V3: Found in all these tissues except medulloblastomas (PubMed:8627343).
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$ 149.00
$ 499.00
Cat# AGI1743
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