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KD-Validated Anti-USO1 Vesicle Transport Factor Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-USO1 Vesicle Transport Factor Rabbit Monoclonal Antibody AGI1760
    Western blotting analysis using anti-USO1 antibody (Cat#62803). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USO1 antibody (Cat#62803, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-USO1 Vesicle Transport Factor Rabbit Monoclonal Antibody AGI1760
    Western blotting analysis using anti-USO1 antibody (Cat#62803). USO1 expression in wild type (WT) and USO1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-USO1 antibody (Cat#62803, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-USO1 Vesicle Transport Factor Rabbit Monoclonal Antibody AGI1760
    Flow cytometric analysis of USO1 expression in HepG2 cells using anti-USO1 antibody (Cat#62803, 1:2,000). Green, isotype control; red, USO1.
    detail
  • ICC - KD-Validated Anti-USO1 Vesicle Transport Factor Rabbit Monoclonal Antibody AGI1760
    Immunocytochemical staining of HepG2 cells with anti-USO1 antibody (Cat#62803, 1:1,000). Nuclei were stained blue with DAPI; USO1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O60763
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1905
Calculated MW Predicted, 108 kDa , observed , 108 kDa
Gene Name USO1
Aliases USO1; USO1 Vesicle Transport Factor; TAP; VDP; P115; General Vesicular Transport Factor P115; Transcytosis Associated Protein; USO1 Homolog, Vesicle Docking Protein (Yeast); USO1 Vesicle Docking Protein Homolog (Yeast); USO1 Vesicle Docking Protein Homolog; Transcytosis-Associated Protein; Vesicle Docking Protein P115; Vesicle Docking Protein; Vesicle-Docking Protein; Protein USO1 Homolog
Immunogen A synthesized peptide derived from human USO1
Additional Information
Gene ID 8615
Other Names General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP
Protein Information
Name USO1
Synonyms VDP
Function General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Cellular Location Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1760
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