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KD-Validated Anti-EEF2K Rabbit Polyclonal Antibody

Rabbit polyclonal antibody

     
  • WB - KD-Validated Anti-EEF2K Rabbit Polyclonal Antibody AGI1776
    Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-EEF2K Rabbit Polyclonal Antibody AGI1776
    Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Eukaryotic elongation factor 2 kinase expression in wild type (WT) and Eukaryotic elongation factor 2 kinase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O00418
Reactivity Rat, Human
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW Predicted, 82 kDa , observed , 105 kDa
Gene Name EEF2K
Aliases EEF2K; Eukaryotic Elongation Factor 2 Kinase; EEF-2K; CaMKIII; EEF-2 Kinase; EC 2.7.11.20; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase; Calmodulin-Dependent Protein Kinase III; Eukaroytic Elongation Factor 2 Kinase; Elongation Factor-2 Kinase; Alternative Protein EEF2K; EC 2.7.11; HSU93850
Immunogen A synthesized peptide derived from human EEF2K
Additional Information
Gene ID 29904
Other Names Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, 2.7.11.20, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K
Protein Information
Name EEF2K
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1776
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