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KD-Validated Anti-PCNA Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-PCNA Mouse Monoclonal Antibody AGI1784
    Western blotting analysis using anti-Proliferating cell nuclear antigen antibody (Cat#62845). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Proliferating cell nuclear antigen antibody (Cat#62845, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-PCNA Mouse Monoclonal Antibody AGI1784
    Western blotting analysis using anti-Proliferating cell nuclear antigen antibody (Cat#62845). Proliferating cell nuclear antigen expression in wild type (WT) and Proliferating cell nuclear antigen shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Proliferating cell nuclear antigen antibody (Cat#62845, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P12004
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB2810
Calculated MW Predicted, 29 kDa , observed , 35 kDa
Gene Name PCNA
Aliases PCNA; Proliferating Cell Nuclear Antigen 2; Cyclin; DNA Polymerase Delta Auxiliary Protein; ATLD2
Immunogen A synthesized peptide derived from human PCNA
Additional Information
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Protein Information
Name PCNA
Function Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Cellular Location Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents
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$ 149.00
$ 499.00
Cat# AGI1784
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