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KD-Validated Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody AGI1790
    Western blotting analysis using anti-Adenylate kinase 1 antibody (Cat#62872). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody AGI1790
    Western blotting analysis using anti-adenylate kinase 1 antibody (Cat#62872). Adenylate kinase 1 expression in wild-type (WT) and adenylate kinase 1 (AK1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenylate kinase 1 antibody (Cat#62872, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • ICC - KD-Validated Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody AGI1790
    Immunocytochemical staining of HepG2 cells with anti-Adenylate kinase 1 antibody(Cat#62872, 1:1,000). Nuclei were stained blue with DAPI; Adenylate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P00568
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1 kappa
Clone Names 24GB2940
Calculated MW Predicted, 45 kDa, observed, 44 kDa
Gene Name AK1
Aliases AK1; Adenylate Kinase 1; Adenylate Monophosphate Kinase; Adenylate Kinase Isoenzyme 1; ATP-AMP Transphosphorylase 1; ATP:AMP Phosphotransferase; EC 2.7.4.3; Myokinase; Testis Secretory Sperm Binding Protein Li 58j; Epididymis Secretory Sperm Binding Protein; EC 2.7.4.10; EC 2.7.4.6; HTL-S-58j; EC 2.7.4; AK 1
Immunogen Recombinant protein of human Adenylate Kinase 1
Additional Information
Gene ID 203
Other Names Adenylate kinase isoenzyme 1 {ECO:0000255|HAMAP-Rule:MF_03171}, AK 1 {ECO:0000255|HAMAP-Rule:MF_03171}, 2.7.4.3, 2.7.4.4, 2.7.4.6 {ECO:0000255|HAMAP-Rule:MF_03171, ECO:0000269|PubMed:23416111}, ATP-AMP transphosphorylase 1 {ECO:0000255|HAMAP-Rule:MF_03171}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03171}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03171}, Myokinase {ECO:0000255|HAMAP-Rule:MF_03171}, AK1 {ECO:0000255|HAMAP-Rule:MF_03171, ECO:0000312|HGNC:HGNC:361}
Protein Information
Name AK1 {ECO:0000255|HAMAP-Rule:MF_03171, ECO:0000312|HGNC:HGNC:361}
Function Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) (PubMed:21080915, PubMed:23416111, PubMed:2542324). Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P05081}.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1790
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