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KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody AGI1798
    Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
    detail
  • WB - KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody AGI1798
    Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#63144). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#63144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody AGI1798
    Flow cytometric analysis of Trophoblast glycoprotein expression in HAP-1 cells using anti-Trophoblast glycoprotein antibody (Cat #62762, 1:2,000). Green, isotype control; red, Trophoblast glycoprotein.
    detail
  • ICC - KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody AGI1798
    Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#63144, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P26358
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB5135
Calculated MW Predicted, 183 kDa, o bserved , 200 k Da
Gene Name DNMT1
Aliases DNMT1; DNA Methyltransferase 1; CXXC9; MCMT; DNMT; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; AIM; ADCADN; HSN1E; Dnmt1
Immunogen A synthesized peptide derived from human Dnmt1
Additional Information
Gene ID 1786
Other Names DNA (cytosine-5)-methyltransferase 1, Dnmt1, 2.1.1.37, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Protein Information
Name DNMT1
Synonyms AIM, CXXC9, DNMT
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS- mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Cellular Location Nucleus. Note=Localized to the perinucleolar region.
Tissue Location Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1798
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