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KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody AGI1808
    Western blotting analysis using anti-Growth associated protein 43 antibody (Cat#AGI1808). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Growth associated protein 43 antibody (Cat#AGI1808, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody AGI1808
    Western blotting analysis using anti-Growth associated protein 43 antibody (Cat#AGI1808). Growth associated protein 43 expression in wild type (WT) and growth associated protein 43 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Growth associated protein 43 antibody (Cat#AGI1808, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody AGI1808
    Flow cytometric analysis of Growth associated protein 43 expression in HAP-1 cells using Growth associated protein 43 antibody (Cat#AGI1808, 1:2,000). Green, isotype control; red, Growth associated protein 43.
    detail
  • ICC - KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody AGI1808
    Immunocytochemical staining of HAP-1 cells with Growth associated protein 43 antibody (Cat#AGI1808, 1:1,000). Nuclei were stained blue with DAPI; Growth associated protein 43 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P17677
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 1195
Calculated MW Predicted, 25 kDa , observed, 4 8 kDa
Gene Name GAP43
Aliases GAP43; Growth Associated Protein 43; Axonal Membrane Protein GAP-43; Neural Phosphoprotein B-50; Neuromodulin; GAP-43; B-50; PP46; Neuron Growth-Associated Protein 43; Nerve Growth-Related Peptide GAP43; Calmodulin-Binding Protein P-57; Protein F1; Growth-Associated Protein 43; Pp46
Immunogen A synthesized peptide derived from human GAP43
Additional Information
Gene ID 2596
Other Names Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43
Protein Information
Name GAP43
Function This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Synapse Cell projection, filopodium membrane; Peripheral membrane protein. Perikaryon {ECO:0000250|UniProtKB:P07936}. Cell projection, dendrite {ECO:0000250|UniProtKB:P07936}. Cell projection, axon {ECO:0000250|UniProtKB:P07936}. Cytoplasm {ECO:0000250|UniProtKB:P07936}. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
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$ 149.00
$ 499.00
Cat# AGI1808
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