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KD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody AGI1835
    Western blotting analysis using anti-G3BP1 antibody (Cat#63223). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP1 antibody (Cat#63223, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody AGI1835
    Western blotting analysis using anti-G3BP1 antibody (Cat#63223). G3BP1 expression in wild type (WT) and G3BP1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G3BP1 antibody (Cat#63223, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody AGI1835
    Flow cytometric analysis of G3BP1 expression in HepG2 cells using anti-G3BP1 antibody (Cat#63223, 1:2,000). Green, isotype control; red, G3BP1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q13283
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4580
Calculated MW Predicted, 52 kDa, observed, 68 kDa
Gene Name G3BP1
Aliases G3BP1; G3BP Stress Granule Assembly Factor 1; G3BP; HDH-VIII; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GTPase Activating Protein (SH3 Domain) Binding Protein 1; Ras GTPase-Activating Protein-Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; RasGAP-Associated Endoribonuclease G3BP; GAP Binding Protein; EC 3.6.4.12; EC 3.6.4.13; HDH VIII; EC 3.6.1
Immunogen A synthesized peptide derived from human G3BP
Additional Information
Gene ID 10146
Other Names Ras GTPase-activating protein-binding protein 1, G3BP-1, 3.6.4.12, 3.6.4.13, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1 {ECO:0000303|PubMed:23279204, ECO:0000312|HGNC:HGNC:30292}
Protein Information
Name G3BP1 {ECO:0000303|PubMed:23279204, ECO:0000312|HGNC:HGNC:30292}
Function Protein involved in various processes, such as stress granule formation and innate immunity (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:30510222, PubMed:30804210). Plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:35977029, PubMed:36183834, PubMed:36279435, PubMed:36692217, PubMed:37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:36279435, PubMed:37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:36279435, PubMed:36692217). Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed:30510222, PubMed:30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed:30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed:34779554). Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed:30804210). May also act as a phosphorylation- dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).
Cellular Location Cytoplasm, cytosol. Perikaryon {ECO:0000250|UniProtKB:P97855}. Cytoplasm, Stress granule. Nucleus Note=Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610)
Tissue Location Ubiquitous..
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1835
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