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KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody AGI1837
    Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#AGI1837). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#AGI1837, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody AGI1837
    Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#AGI1837). DAZ associated protein 1 expression in wild-type (WT) and DAZ associated protein 1 (DAZAP1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#AGI1837, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody AGI1837
    Flow cytometric analysis of DAZ associated protein 1 expression in HepG2 cells using anti-DAZ associated protein 1 antibody (Cat#AGI1837, 1:2,000). Green, isotype control; red, DAZ associated protein 1.
    detail
  • ICC - KD-Validated Anti-DAZ Associated Protein 1 Rabbit Monoclonal Antibody AGI1837
    Immunocytochemical staining of HepG2 cells with anti-DAZ associated protein 1 antibody(Cat#AGI1837, 1:1000). Nuclei were stained blue with DAPI; DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q96EP5
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4600
Calculated MW Predicted, 43 kDa, observed, 43 kDa
Gene Name DAZAP1
Aliases DAZAP1; DAZ Associated Protein 1; Deleted In Azoospermia-Associated Protein 1; DAZ-Associated Protein 1; MGC19907; Deleted In Azoospermia Associated Protein 1; Testicular Tissue Protein Li 50
Immunogen Recombinant protein of human DAZAP1
Additional Information
Gene ID 26528
Other Names DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1
Protein Information
Name DAZAP1
Function RNA-binding protein, which may be required during spermatogenesis.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm (By similarity)
Tissue Location Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1837
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