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KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody AGI1854
    Western blotting analysis using anti-cyclin E1 antibody (Cat#AGI1854). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E1 antibody (Cat#AGI1854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody AGI1854
    Western blotting analysis using anti-cyclin E1 antibody (Cat#AGI1854). Cyclin E1 expression in wild-type (WT) and cyclin E1 (CCNE1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E1 antibody (Cat#AGI1854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody AGI1854
    Flow cytometric analysis of Cyclin E1 expression in HeLa cells using anti-Cyclin E1 antibody (Cat#AGI1854, 1:2,000). Green, isotype control; red, Cyclin E1.
    detail
  • ICC - KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody AGI1854
    Immunocytochemical staining of Hela cells with cyclin E1 antibody (Cat#AGI1854, 1:1,000). Nuclei were stained blue with DAPI; Cyclin E1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P24864
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4745
Calculated MW Predicted, 47 kDa, observed, 47 kDa
Gene Name CCNE1
Aliases CCNE1; Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1
Immunogen A synthesized peptide derived from human Cyclin E1
Additional Information
Gene ID 898
Other Names G1/S-specific cyclin-E1, CCNE1, CCNE
Protein Information
Name CCNE1
Synonyms CCNE
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1854
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