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KD-Validated Anti-HRAS Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-HRAS Mouse Monoclonal Antibody AGI1910
    Western blotting analysis using anti-HRAS antibody (Cat#63452). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HRAS antibody (Cat#63452, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-HRAS Mouse Monoclonal Antibody AGI1910
    Western blotting analysis using anti-HRAS antibody (Cat#63452). HRAS expression in wild type (WT) and HRAS shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HRAS antibody (Cat#63452, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P01112
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB5695
Calculated MW Predicted, 21 kDa, Observed, 18 kDa
Gene Name HRAS
Aliases HRAS; HRas Proto-Oncogene, GTPase; HRAS1; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; Harvey Rat Sarcoma Viral Oncogene Homolog; Transforming Protein P21; GTPase HRas; P21ras; Ras Family Small GTP Binding Protein H-Ras; Harvey Rat Sarcoma Viral Oncoprotein; Transformation Gene: Oncogene HAMSV; GTP- And GDP-Binding Peptide B; Ha-Ras1 Proto-Oncoprotein; C-Has/Bas P21 Protein; P19 H-RasIDX Protein; EC 3.6.5.2; C-BAS/HAS; C-HA-RAS1; H-RASIDX; C-H-RAS; H-Ras-1; C-H-Ras; Ha-Ras; HAMSV; RASH1; CTLO
Immunogen Recombinant protein of human HRAS
Additional Information
Gene ID 3265
Other Names GTPase HRas, 3.6.5.2, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Protein Information
Name HRAS
Synonyms HRAS1
Function Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P20171}; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
Tissue Location Widely expressed..
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1910
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