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KD-Validated Anti-PFKP Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-PFKP Mouse Monoclonal Antibody AGI1936
    Western blotting analysis using anti-PFKP antibody (Cat#AGI1936). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKP antibody (Cat#AGI1936, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PFKP Mouse Monoclonal Antibody AGI1936
    Western blotting analysis using anti-PFKP antibody (Cat#AGI1936). PFKP expression in wild type (WT) and PFKP knockdown (KD) HSHC cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PFKP antibody (Cat#AGI1936, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PFKP Mouse Monoclonal Antibody AGI1936
    Flow cytometric analysis of PFKP expression in HepG2 cells using anti-PFKP antibody (Cat#AGI1936, 1:2,000). Green, isotype control; red, PFKP.
    detail
  • ICC - KD-Validated Anti-PFKP Mouse Monoclonal Antibody AGI1936
    Immunocytochemical staining of HepG2 cells with anti-PFKP antibody (Cat#AGI1936, 1:1,000). Nuclei were stained blue with DAPI; PFKP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q01813
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB6380
Calculated MW Predicted, 86 kDa, observed, 75 kDa
Gene Name PFKP
Aliases PFKP; Phosphofructokinase, Platelet; PFK-C; PFKF; ATP-Dependent 6-Phosphofructokinase, Platelet Type; Phosphofructo-1-Kinase Isozyme C; 6-Phosphofructokinase Type C; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-P; 6-Phosphofructokinase, Platelet Type; Phosphofructokinase, Platelet Type; Phosphofructokinase 1; EC 2.7.1
Immunogen Recombinant protein of human PFKP
Additional Information
Gene ID 5214
Other Names ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-P, 2.7.1.11 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKP, PFKF
Protein Information
Name PFKP
Synonyms PFKF
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1936
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