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KD-Validated Anti-DNAJB1 mouse monoclonal antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-DNAJB1 mouse monoclonal antibody AGI1965
    Western blotting analysis using anti-DNAJB1 antibody (Cat#AGI1965). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJB1 antibody (Cat#AGI1965, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-DNAJB1 mouse monoclonal antibody AGI1965
    Western blotting analysis using anti-DNAJB1 antibody (Cat#AGI1965). DNAJB1 expression in wild type (WT) and DNAJB1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNAJB1 antibody (Cat#AGI1965, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P25685
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 24GB6700
Calculated MW Predicted, 38 kDa, observed, 38 kDa
Gene Name DNAJB1
Aliases DNAJB1; DnaJ Heat Shock Protein Family (Hsp40) Member B1; RSPH16B; Hsp40; HSPF1; Sis1; DnaJ (Hsp40) Homolog, Subfamily B, Member 1; DnaJ Homolog Subfamily B Member 1; Heat Shock 40 KDa Protein 1; DnaJ Protein Homolog 1; Human DnaJ Protein 1; Radial Spoke 16 Homolog B (Chlamydomonas); Radial Spoke 16 Homolog B; Heat Shock Protein 40; DNAJ1; HSP40; HDj-1; Hdj1; HDJ1
Immunogen Recombinant protein of human DNAJB1
Additional Information
Gene ID 3337
Other Names DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1, HSPF1
Protein Information
Name DNAJB1
Synonyms DNAJ1, HDJ1, HSPF1
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1965
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