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KD-Validated Anti-Glucose-6-phosphate dehydrogenase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Glucose-6-phosphate dehydrogenase Rabbit Monoclonal Antibody AGI1968
    Western blotting analysis using anti-Glucose-6-phosphate dehydrogenase antibody (Cat#63652). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucose-6-phosphate dehydrogenase antibody (Cat#63652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Glucose-6-phosphate dehydrogenase Rabbit Monoclonal Antibody AGI1968
    Western blotting analysis using anti-Glucose-6-phosphate dehydrogenase antibody (Cat#63652). Glucose-6-phosphate dehydrogenase expression in wild type (WT) and glucose-6-phosphate dehydrogenase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glucose-6-phosphate dehydrogenase antibody (Cat#63652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Glucose-6-phosphate dehydrogenase Rabbit Monoclonal Antibody AGI1968
    Flow cytometric analysis of Glucose-6-phosphate dehydrogenase expression in HepG2 cells using Glucose-6-phosphate dehydrogenase antibody (Cat#63652, 1:2,000). Green, isotype control; red, Glucose-6-phosphate dehydrogenase.
    detail
  • ICC - KD-Validated Anti-Glucose-6-phosphate dehydrogenase Rabbit Monoclonal Antibody AGI1968
    Immunocytochemical staining of HepG2 cells with Glucose-6-phosphate dehydrogenase antibody (Cat#63652, 1:1,000). Nuclei were stained blue with DAPI; Glucose-6-phosphate dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 µm.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P11413
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1795
Calculated MW Predicted, 59 kDa , observed, 58 kDa
Gene Name G6PD
Aliases G6PD; Glucose-6-Phosphate Dehydrogenase; G6PD1; Glucose-6-Phosphate 1-Dehydrogenase; EC 1.1.1.49; Epididymis Secretory Sperm Binding Protein
Immunogen A synthesized peptide derived from human G6PD
Additional Information
Gene ID 2539
Other Names Glucose-6-phosphate 1-dehydrogenase, G6PD, 1.1.1.49, G6PD
Protein Information
Name G6PD
Function Catalyzes the rate-limiting step of the oxidative pentose- phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein
Tissue Location Isoform Long is found in lymphoblasts, granulocytes and sperm
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1968
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